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Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
01/09/2015 |
Data da última atualização: |
09/09/2015 |
Autoria: |
SILVA, E. C. da. |
Título: |
Os métodos quantitativos e as tendencias da área de ciência e tecnologia na pesquisa agropecuária. |
Ano de publicação: |
1995 |
Fonte/Imprenta: |
Brasilia: EMBRAPA-DPD / EMBRAPA-SPI, 1995. |
Páginas: |
132p. |
Idioma: |
Português |
Notas: |
Anais do 3. Encontro de Metodos Quantitativos da EMBRAPA e do SNPA, 1995, Brasilia, DF. |
Conteúdo: |
Palestras: Planejamento estrategico/tendencias da area de ciencia e tecnologia e os metodos quantitativos; Aplicacao do enfoque de sistemas em P&D em atendimento as demandas da sociedade; Auditoria da qualidade: controle estatistico da qualidade; Avaliacao dos impactos socio-economicos da pesquisa agropecuaria - ex-ante e ex-post; Sistema de informacao; Avaliacao e medida de qualidade das instituicoes de pesquisa agropecuaria - o caso da EMBRAPA; As tecnicas multivariadas no contexto dos metodos quantitativos; Os metodos quantitativos para a melhoria da qualidade de pesquisa; O ensino de estatistica no contexto dos metodos quantitativos no SNPA; Paineis: A importancia dos metodos quantitativos na pesquisa agropecuaria sob o ponto de vista do gerente de pesquisa; Atividades de modelagem de sistemas nas Unidades de pesquisa; Atividades de estatistica nas Unidades de Pesquisa; Atividade de socio-economia e de sociometria e econometria nas Unidades de Pesquisa. |
Palavras-Chave: |
Analise quantitativa; Encontro; Estatistica; Informacao; Information; Information systems; Metodos quantitativos; Pesquisa; Quantitative analysis; Research; Sistema; Sistemas de informacoes; Statistic; Statistics; System. |
Categoria do assunto: |
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Marc: |
LEADER 01893nam a2200313 a 4500 001 1007813 005 2015-09-09 008 1995 bl uuuu 00u1 u #d 100 1 $aSILVA, E. C. da. 245 $aOs métodos quantitativos e as tendencias da área de ciência e tecnologia na pesquisa agropecuária. 260 $aBrasilia: EMBRAPA-DPD / EMBRAPA-SPI$c1995 300 $a132p. 500 $aAnais do 3. Encontro de Metodos Quantitativos da EMBRAPA e do SNPA, 1995, Brasilia, DF. 520 $aPalestras: Planejamento estrategico/tendencias da area de ciencia e tecnologia e os metodos quantitativos; Aplicacao do enfoque de sistemas em P&D em atendimento as demandas da sociedade; Auditoria da qualidade: controle estatistico da qualidade; Avaliacao dos impactos socio-economicos da pesquisa agropecuaria - ex-ante e ex-post; Sistema de informacao; Avaliacao e medida de qualidade das instituicoes de pesquisa agropecuaria - o caso da EMBRAPA; As tecnicas multivariadas no contexto dos metodos quantitativos; Os metodos quantitativos para a melhoria da qualidade de pesquisa; O ensino de estatistica no contexto dos metodos quantitativos no SNPA; Paineis: A importancia dos metodos quantitativos na pesquisa agropecuaria sob o ponto de vista do gerente de pesquisa; Atividades de modelagem de sistemas nas Unidades de pesquisa; Atividades de estatistica nas Unidades de Pesquisa; Atividade de socio-economia e de sociometria e econometria nas Unidades de Pesquisa. 653 $aAnalise quantitativa 653 $aEncontro 653 $aEstatistica 653 $aInformacao 653 $aInformation 653 $aInformation systems 653 $aMetodos quantitativos 653 $aPesquisa 653 $aQuantitative analysis 653 $aResearch 653 $aSistema 653 $aSistemas de informacoes 653 $aStatistic 653 $aStatistics 653 $aSystem
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Biblioteca Rui Tendinha (BRT) |
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| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
13/01/2015 |
Data da última atualização: |
13/01/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
Thesagro: |
Café; Coffea canephora; Genetics. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03034naa a2200445 a 4500 001 1004907 005 2015-01-13 008 2012 bl uuuu u00u1 u #d 100 1 $aMARRACCINI, P. 245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource] 260 $c2012 520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. 650 $aCafé 650 $aCoffea canephora 650 $aGenetics 653 $aCandidate gene 653 $aCoffea canephora 653 $aDifferential expression 653 $aDrought acclimation 653 $aGenética 653 $aProteomic 653 $aProteômica 653 $aReal time PCR 700 1 $aVINECKY, F. 700 1 $aALVES, G. S. C. 700 1 $aRAMOS, H. J. O. 700 1 $aELBELT, S. 700 1 $aVIEIRA, N. G. 700 1 $aCARNEIRO, F. A. 700 1 $aSUJII, P. S. 700 1 $aALEKCEVETCH, J. C. 700 1 $aSILVA, V. A. 700 1 $aDaMATTA, F. M. 700 1 $aFERRÃO, M. A. G. 700 1 $aLEROY, T. 700 1 $aPOT, D. 700 1 $aVIEIRA, L. G. E. 700 1 $aSILVA, F. R. da 700 1 $aANDRADE, A. C. 773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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